Anything with Samus in bottom and not in low equals auto-fail.
Assuming a reasonably sized low tier, this is my guess on what it will look like:
Low Tier
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Ness: Has a good approaching and spacing tool (fair). PK fire, although slow, sets up for combos. Yo-yos are a good smash attack that leaves him protected. Most powerful throw in the game. However, he has awful recovery and a grab release hurdle.
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Mario: Only okay killing power and small range (not as bad as Sonic), but excellent edgeguarding capabilities, a decent approach, and actual combo potential. He could be mid if he was at least D rank in the tournament listings.
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Sonic: An excellent grab game with a great tech chasing game. Combo potential, good aerials, and the ability to rack up fast damage. Low killing power and horrible range hold him back.
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Pokemon Trainer: Ivysaur has great spacing tools, a good, however limited projectile. Can rack up insane damage with bullet seed and fsmash as well as uair and even a fresh fair are good kill moves. Also has the best matchups of the three. Charizard is a survivalist with good recovery, great weight, being able to survive into high percentages. Rock Smash rocks (lol). Great grab range rivaling King Dedede. Squirtle just sucks. Ivysaur and Charizard would have better tier placement if they could fight on their own.
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Samus: Samus has great tools for spacing (ftilt and zair), a decent camping game (try avoiding homing missle, charge shots and her zair all at once), the best recovery in terms of options aside from multi-jumpers/Wario, combo ability (ALL of her aerials auto-cancel), a great gimp game, the ability to rack up quick damage (not as well as Meta/Sonic), and great weight. Hylian said if she had a good KO move, she would be high tier easily. Honestly, going by character potential, she has the best chance at middle next to Sonic and Mario.
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Yoshi: Bair is a good aerial approach, eggs are a damaging projectile with a rediculous range, pivot grab stops almost all ground approaches (it is actually fast unlike his other grabs), has the fastest air speed, is next to Samus in weight, and his recovery shouldn't ever be gimped due to his second jump having heavy armor frames. I am giving Yoshi an objective analysis and honestly have to say he is better than the guys below him. He would probably move above Pokemon Trainer if he has an even matchup against Meta Knight.
-Bowser: He has a good ground game, an excellent ftilt (obviously behind Snake's), two great croud clearing moves in Dsmash and Up-B, and is very heavy. Has a bad approach however, has bad aerials aside from uair and fair and even these two are pretty laggy. Predictable recovery, and is very easily juggled.
Bottom tier
Ike
Link
Jigglypuff
Ganondorf
Captain Falcon