Might as well go over the other two sides too
The Disney side has Mickey Mouse, Minnie Mouse, Donald Duck, Daisy Duck, Goofy, Pluto, Pete, Horace Horsecollar, Clarabelle Cow, Chip and Dale (Utilizing gadgets and vehicles from Rescue Rangers), Mortimer Mouse, Scrooge McDuck (with the Triplets and Webby), The Big Bad Wolf (from the Silly Symphonies shorts), The Phantom Blot, Oswald the Lucky Rabbit, Snow White, King Mickey, Dawkwing Duck, José Carioca, Panchito Pistoles, Roget Rabbit, and Yen Sid.
The Looney Tunes side has Bug Bunny, Lola Bunny, Daffy Duck, Tina Russo, Porky Pig, Foghorn Leghorn, Elmer Fudd, Yosemite Sam, Taz, Marvin the Martian, Sylvester and Tweety, Wile E. Coyote, the Roadrunner, Gossamer, Bosko, Pepé Le Pew, Super-Rabbit, Duck Dodgers, Speedy Gonzales, Hippity Hopper, The Warner Bros. (and the Warner Sister, Dot!), and Sam Sheepdog.
A few characters I had considered were Bluto, Bimbo the Dog, the Fliescher Studios Superman, Koko the Clown, Pinky and the Brain, Peter Pan, and Tom and Jerry's Spike. But they didn't quite make it for a handful of reasons.