Here's the next tally, approximately 50 voters more than the previous one, for a total of 214. Since we hit the 200 mark, I'll add a little extra analysis on this one.
As usual, it'll be this format, and I've only included characters with at least 10 votes:
Name: Score (+1s, +1/2s, -1s, %)
A Tier(s) (really, each of these characters are in a tier of their own)
Isaac: 115 (114, 22, 10, 93.15%)
Ridley: 90 (96, 32, 22, 85.33%)
Lyn: 63.5 (73, 23, 21, 81.21%)
Ganondorf/Black Shadow: 56.5 (79, 21, 33, 75.19%)
B Tier
Dark Samus: 40.5 (45, 33, 21, 78.79%)
King K. Rool: 37 (34, 32, 13, 83.54%)
Skapon: 34.5 (39, 27, 18, 78.57%)
Saki: 30 (27, 24, 9, 85.00%)
Bowser Jr.: 29 (32, 26, 16, 79.38%)
Masked Man/Claus: 27.5 (24, 13, 3, 92.50%)
C Tier
Skull Kid: 21.5 (17, 15, 3, 91.43%)
Dixie Kong: 20 (30, 36, 28, 70.21%)
Paper Mario: 18.5 (22, 31, 19, 73.61%)
Sami: 16.5 (27, 13, 17, 70.18%)
Tom Nook: 16.5 (15, 19, 8, 80.95%)
Andy: 14 (13, 18, 8, 79.49%)
Samurai Goroh: 12.5 (12, 23, 11, 76.09%)
Ray: 12 (6, 12, 0, 100%)
D Tier
Toad: 3.5 (17, 15, 21, 60.38%)
Tetra: 2.5 (9, 11, 12, 62.50%)
Waluigi: 2 (23, 26, 34, 59.04%)
Hector: 1.5 (10, 17, 17, 61.36%)
Red/Pokemon Trainer: 1.5 (4, 3, 4, 64.64%)
E Tier
Plusle & Minun: -4 (5, 8, 13, 50.00%)
Krystal: -7.5 (10, 25, 30, 53.85%)
Toon Zelda: -10.5 (6, 5, 19, 36.67%)
Pichu: -12 (31, 22, 54, 49.53%)
Sonic Tier
Tails: -26.5 (13, 17, 48, 38.46%)
Knuckles: -30.5 (11, 9, 46, 30.30%)
Shadow: -51.5 (11, 7, 66, 21.43%)
Unpopularity Contest
Since it's pretty easy to see how well characters are doing on the positive end, here's the flip side, ordering them from worst to least by number of negative votes only:
Shadow - 66
Pichu - 54
Tails - 48
Knuckles - 46
Waluigi - 34
Ganondorf/Black Shadow - 33
Krystal - 30
Dixie - 28
Ridley - 22
Dark Samus - 21
Lyn - 21
Toad - 21
Paper Mario - 19
Skapon - 18
Sami - 17
Hector - 17
Bowser Jr - 16
Plusle & Minun - 13
King K. Rool - 13
Tetra - 12
Samurai Goroh - 11
Isaac - 10
Saki - 9
Andy - 8
Tom Nook - 8
Red - 4
Masked Man - 3
Skull Kid 3
Ray - 0
(Note most of the ones toward the end were late entries)
Trends since last tally
-Ganondorf & Black Shadow made a huge leap ahead and jumped out of B tier into their own tier.
-Dark Samus made another big jump to reach 5th place.
-Masked Man fell behind as most of the other characters in its tier jumped ahead.
-Dixie made a little jump while Samurai Goroh fell behind.
-Plusle & Minun lagged so far behind that it dropped a tier, while Toad and Waluigi made huge jumps to bring them out of the negatives.
-Ray is now on the board and doing fairly well.
Thoughts on specific characters
Isaac - Still without contest (though Ridley has been inching much closer). Although this poll is completely unofficial, I think he makes the strongest candidate for most wanted, easily.
Ridley, Dark Samus - Only one of these two will probably get in. But I think there's an extremely strong chance that one of them will (assuming more characters will be added). Ridley has the strong popularity and desire to be added ever since Melee, although he is more difficult. Dark Samus, on the other hand, is much easier to implement as a clone and so is Ridley's backup. Major note: MANY votes were contingent on Ridley making it. So if Ridley was ruled out for some reason, Dark Samus' votes would be much higher. Again, I just want to emphasize that the votes of these two are interconnected and interdependent.
Lyn - With 75% of her voters in favor of her, and amassing exactly 100 voters in support, she's a very strong candidate.
Ganondorf/Black Shadow - Despite the vocal hatred for this set, it's continued to rise. There have been recent movements to suggest making Ganondorf just have an extra stance (similar to Samus) that would allow him to use his sword. That is one option, although that changes the character significantly. It has also been alleged several times in this thread that almost none of the Ganon/BS voters actually care about the Black Shadow portion. I'm going to say this right now that is wrong -- there have been quite a few members who explicitly stated they wanted that F-Zero character, and some saying they voted for it BECAUSE of Black Shadow, not because of Ganondorf. Finally, this entry is a big asterisk because I believe Ganondorf mains should determine how much of a possibility this option since their character would actually get changed (although just aesthetically), whereas other characters would be added from thin air and not affect any current players.
King K. Rool - With 66 votes in favor, 13 against, and 83.54% of his voters supporting him, he's a pretty strong candidate.
Skapon - With 66 votes in favor, 18 against and 78.6% of his voters supporting him, he's a pretty strong candidate.
Sami, Andy - Again, these are two characters that are interconnected, with some votes specifically saying they voted for Sami because she was currently ranked higher. I will say this right now: I am EXTREMELY against this kind of voting, because this poll is supposed to reflect opinion and NOT be a ranking of preference. Each character needs to be treated individually, but because there are some characters like these... I just have to point them out.
Red - I added him to the poll relatively recently because of a little surge of talk in the thread.. but he's really failed to impress at all. Only 11 votes total still. Ray has had a recent surge and already has accumulated 18 votes. Likewise, Skull Kid and Tetra were added to the OP at the same time as Red and have many, many more votes.
Masked Man - While not nearly as bad as Red, he also similarly dried up after his huge surge to the "B tier." After the surge, there have been practically no votes for him since, which let him drop to the bottom of that tier. Perhaps his popularity might be pretty niche as ChronoBound previously suggested.
Pichu - Ugh. Look up controversial in the dictionary and you'll see Pichu. Responsible for messing the discussion MULTIPLE times in this thread, but anyways. As it stands, Pichu is < 50%, which is way below the average support (somewhere around 80%), and is fact only better than Toon Zelda, and the three Sonic characters. Objectively, Pichu is a terrible candidate, and so far it's mainly its role as a veteran keeping it in the game. (Although I'm pretty sure many people will respond with their subjective thoughts)
Sonic characters - I think the numbers speak for themselves. They are the opposite of Isaac, and the numbers show that overwhelmingly, it would be a very bad idea to include any of them.
All the others in between - Did not mention because it doesn't seem like there is strong enough support or dislike for them compared to the two ends of the spectrum. Though Saki, Bowser Jr., and Masked Man are other favored characters, while Plusle & Minun, Krystal, and Toon Zelda are other unfavorable characters.