I made some minor changes to my roster.
I took off Amy Rose because I don't really like her
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My reasoning for each character.
Shantae is one of the female third party characters that are closest to Nintedo, and she would make an awesome fit.
Villager's female counterpart not much explanation her other than she's the feminine mascot of Animal crossing.
Pikachu would be a perfect fit but I feel that adding Pichu in its place adds the extra flair to the sisters group.
Impa is the second most important non-Zelda female in the series but I merged her with Zelda to add room for Tetra and Twinrova.
Anna as the female mascot of Fire Emblem seems like a perfect fit for this kind of roster game.
Sami happens to be the most popular female Advanced Wars character and she's cool and represents a different series.
Ribbon's slot was actually meant for Drawcia but I couldn't find her so I put Kirby's love interest instead.
Barbara has her own series so as one of the few females with her own series she's a perfect fit.
Rosalina is probably the second most important female in the Mario universe, and she's also in Mario spin offs.
Tetra to relate to Zelda in a similar way that Link and Toon Link do each other. Instead of Toon Zelda I picked Tetra because she looks more interesting.
Lyn was picked to represent the female lords, I was going to pick Lucina but I bought into this.
Krystal. When I think of a female Starfox icon I automatically think Krystal.
Lip is probably the most popular retro female character.
Brittany, like Krystal, when I think of Pikmin's female mascot I think Brittany.
Cynthia is a popular wildcard, she can be that one female trainer.
Dixie is one of the most iconic Nintendo females and a perfect fit for a roster like this.
Twinrova isn't the typical anyone would pick, but I see her as the female Ganondorf and as a rare fit as one of the few female baddies.
Kumatora, I had a hard time picking between Paula and Kumatora but Kumatora's pink hair stands out in her favor.
Jody, like Krystal or Brittany, when I think of a female mascot of F-zero I automatically think Jody and she seems like a badass.
Jill placed right under the pokemon girls because she was created by the same developers. She's also one of the few girls with their own game.
Pauline for being one of the oldest characters to appear in modern games, to represent the start of Mario & DK, and to rival Peach.
Palutena like Dixie is probably one of the most popular and iconic Nintendo females and the Kid Icarus female mascot.
Jenna is chosen to be the female mascot of Golden Sun although i'm not very sure if she is the most important female in GS.
Mona the more iconic Wario Ware female character she also seems to be a popular and well liked character and I really like her song.
Medusa similar to Twinrova she's a rare female villain and also important as Palutena's rival.