Well, I contemplated the possibility that in the end they did not appear in the game, even as AT, we'll have to wait and see if Sakurai. We'll have to wait if Sakurai includes them in the DLC, as we could see, Kat & Ana appears in the game as Spirits, i hope that does not take away his chances for the DLCs.
By the way, honestly the only thing i liked about the direct, was the inclusion of the Piranha plant. even being an deliberate troll character, the moveset & animations are hillarious and very original, i'm sure nobody saw it coming (I think not even sakurai), in fact, the plant will be my main character in the game
for the simple reason that it is very original for the game.
I know a lot of people are disgusted and angry with Sakurai due to the actual situation about the characters they wanted (Like the deconfirmation of Issac, Ashley, Waluigi, Shadow, Etc. being assist trophies, or maybe the possible non-inclusion of the characters they want due to they are actually spirits), i already saw that the possibilities of entering Kat and Ana were few, but personally i'm very satisfied with the actual content of the game.